/ 'from a practical and good in fact. Bacteria are divided into two natural groups depending on their cell wall structure. Some are painted by Gram stain and are called gram-positive strattera prescription, others not retain color in the process of bleaching and called Gram-negative. To verify that V. and A. tumefaciens ziYShz is gram positive or gram-negative using the Gram staining method. (Stains:
PPPPPPPP. Crystal Violet solution (0 5% aqueous)
(1)
PPPPPPPPTake microscope slide using a pipette and place two or
PPPPPPPPthree drops of ethanol on a slide . Using a clean cloth
PPPPPPPPwipe slides. Set IV in drop dispenser (2).
PPPPPPPPHaving put on some goggles, light Bunsen
PPPPPPPPburner. Take the loop and flame it. After as burning
PPPPPPPPthe wire loop, immerse it in some of the cultural and B. Subtilis
PPPPPPPPspread on the slide to form a smear (3).
PPPPPPPPHolding slide one side, flip and slide pass
PPPPPPPPthe slide through the flame very quickly, while a sample
PPPPPPPPdry. This method is known as "thermal fixation". >> << slide PPPPPPPPshould then allowed to cool to room temperature. (4)
PPPPPPPPNext coverage smear some crystal violet solution. PPPPPPPPAllow dab on the stain for 1 minute, then rinse
PPPPPPPPwater (from water bottles). (5)
PPPPPPPPUsing iodine solution, put a few drops of it on PPPPPPPPsmear >>. << let it fix within a minute, then rinse
PPPPPPPPsome ...
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